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. 2009 Nov 30;4(11):e8047. doi: 10.1371/journal.pone.0008047

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Santos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Stimulating (cell-attached) and recording (perforated-patch) pipettes were placed on the soma of an SG neuron. Stimulation (1 ms) increased with a 10 nA increment. Subthreshold, the membrane responses were considered as passive. Threshold, the AIS and AH+S components could be best distinguished (transitions are indicated by arrowheads). The AIS component was initiated within the 1 ms of the stimulation. The passive response was multiplied by the corresponding scaling factor. Threshold+10 nA, the interval between the AIS and AH+S components became shorter. The AIS component was completely generated within the 1 ms of the stimulation. Two SG neurons shown have different proportions between the amplitudes of the AIS and AH+S components and different kinetics of transitions. Horizontal grey lines, 0 mV. Resting potentials were about −70 mV.