Figure 7. Effect of transient transgenic expression of WT or mutant apoL1 on trypanosome infection in mice.

A. Detection of WT apoL1 at days 1, 4 and 8 after hydrodynamic injection of the plasmid construct, monitored by incubation of Western blots of mouse serum proteins with rat anti-apoL1 antibodies. Mice 1 and 2 were injected with control (empty) plasmid. The lane labelled NHS shows the result obtained with normal human serum. B. Detection of the different apoL1 variants at day 1 post-injection of DNA (CTRL = control empty plamid). The mice whose sera were analyzed here were used for the trypanosome infection experiments reported in panel C (S = T. b. rhodesiense ETat 1.2S; R = T. b. rhodesiense ETat 1.2R; g = T. b. gambiense LiTat 1.2). Normal human serum (NHS) was used for comparison. Loading control by Ponceau red staining of albumin is shown below each panel. C. 24 h post-injection of DNA, 10⁶ parasites of the indicated strains were inoculated intraperitoneally into mice. Parasitemia was measured 3 days after parasite inoculation (control: empty plamid).