Figure 3.—

The mean grain hardness difference between lines homozygous positive or negative for individual PIN mutations relative to their location. Asterisks indicate where the hardness difference between an individual Pin mutant and its wild-type group was significant. The open boxed area denotes the signal peptide region while the shaded/solid boxed region denotes mature peptide sequence with the solid boxed region indicating the tryptophan-rich loop region. The positions of cysteines are denoted by C's. The position of the amplification primers is as shown.