Figure 4.—

Multispecies PINA and PINB protein alignment. Polypeptide primary sequences from representative diploid wheat relatives (see Table 2) were aligned using Clustal W (Thompson et al. 1994). Identical residues are shaded. Points of mutation that generate hardness differences >20 units from wild type are marked by an asterisk. For PINA these are P63S, G75S, L87F, and C132Y. For PINB these are D63N, T67I, G75D, E80K, and C85Y. The signal peptide is marked in italics. The tryptophan box loop region is underlined.