TABLE 1.

Pin mutation frequency and density in Alpowa EMS-induced population

	Pina	Pinb	Both Pins
Mutation frequency via direct phenotyping and sequencinga
Missense	37	31	68
Nonsense	11	12	23
Silent	23	34	57
Total	71	77	148
Mutation frequency via sequencingb
Missense	33	27	60
Nonsense	6	9	15
Silent	23	32	55
Total	62	68	130
Mutation densityc	1/12 kb	1/11 kb	1/11.5 kb
Observed mutation class ratios
Missense	0.53	0.4	0.46
Nonsense	0.1	0.13	0.12
Silent	0.37	0.47	0.42
Expected mutation class ratiosd
Missense	0.56	0.55	0.56
Nonsense	0.1	0.11	0.1
Silent	0.34	0.34	0.34
Chi-square P-valuee	0.52	<0.01	<0.01

^aTotal cumulative mutation frequency found via phenotyping of 630 and direct sequencing of 1700 M₂ Alpowa lines.

^bThe frequency of mutations found by direct sequencing of 1700 M₂ Alpowa lines.

^cThe mutation density was calculated using the frequency of mutations found via direct sequencing of 1700 M₂ Alpowa lines.

^dExpected ratio of three types of mutations from total was calculated using all potential nucleotide substitutions expected from EMS-induced transition mutations (G to A or C to T).

^eP-values are from chi-square tests that were used to compare the number of observed and expected mutation types.