TABLE 3.
The effect of missense mutations occurring in both PINA and PINB on grain hardness
| Region | Allele | Hardness change (D1x–D1a)a | Hardness change PINBx–PINAxb |
|---|---|---|---|
| Signal peptide | PINA V24I | 0.52 | −3.24 |
| PINB V24I | −2.72 | ||
| PINA G26D | −3.00 | 2.21 | |
| PINB G26D | −0.79 | ||
| Trp domain | PINA D61N | 5.46 | 18.48* |
| PINB D63N | 23.94 | ||
| PINA T65I | 0.61 | 28.56* | |
| PINB T67I | 29.17 | ||
| PINA E78K | 1.9 | 45.2* | |
| PINB E80K |
47.10 |
*Denotes significance in comparisons of the grain hardness change between the identical mutation in PINA and PINB at P < 0.05.
a
Difference in grain hardness in comparisons of two independently derived seed composites, where each seed composite was homozygous positive (D1x) or negative (D1a) for a Pin mutation.
b
Difference in impact of individual identical mutations between PINA and PINB upon grain hardness.