Figure 2.—

Mnr2 is a vacuolar membrane protein. BY4743 cells expressing YFP-Mnr2 from a low-copy vector (YCpCitMNR2) or transformed with an empty vector (pFL38, control) were grown for 6 hr in LMM + 1 mm Mg (high Mg) or in LMM + 1 μm Mg (low Mg). Cells were treated with FM4-64 to visualize the vacuole membrane, and images were captured with the appropriate filter set (FM4-64 or YFP). Signal overlap is shown (Merge). DIC, differential interference contrast.