Figure 5.—

Retrograde mutant bulges contain some but not all flagellar proteins. (A and B) Phase contrast of bulges (arrowheads) on fla15-1 cells (A) and their absence in the rescued fla15-1∷IFT144 strain (B). In the mutant strain, >80% of the cells have bulges (n = 200) and no bulges were observed in the rescued strains. (C–E) Phase images of cells with bulges. (F–H) Immunofluorescence of cells with bulges. (F) p28 antibody. (G) IFT74/72 antibody. (H) α-Tubulin antibody. IFT74/72 is present in the bulges, but α-tubulin and p28 are not.