Table 2. PCR primers for insertion screening and marker genotyping.
| Feature | Sequence type | CFA15 location (Mb) | Forward primer (5′>3′) | Reverse primer (5′>3′) | Reference |
|---|---|---|---|---|---|
|
RPGRIP1_insertion* |
Gene |
21.34 |
CTTAAGGAGAACACAAGGTAC |
GAAGAGCACATGTTGGTGAAGG |
[19] |
| CAMC15.001 |
Microsatellite |
16.74 |
TTCGCTTCCTCCCTCACATG |
TGAGCTGCAGACAAAGGCC |
- |
| CAMC15.036 |
Microsatellite |
17.32 |
CCTGTGTGGCAGCAGTTGAAT |
ACATTGGGTTCCGCATTCAGT |
- |
| CAMC15.037 |
Microsatellite |
18.13 |
TTTCCAACCTCCCTCCAACC |
TGGCGCGGTGGTTTAGCAT |
- |
| CAMC15.038 |
Microsatellite |
18.76 |
GCTACATGTCAGGCGTTGTGT |
TCCTCGTCTCTACAGTGGGCT |
- |
| CAMC15.006 |
Microsatellite |
19.48 |
CCCGCACCACATGCTCTC |
AGCTACGTCAGCTTCTCTAC |
- |
| CAMC15.039 |
Microsatellite |
19.87 |
AACGCTTAGCTTGCTTCCACG |
CATCATCGGGGAAACCCAAGT |
- |
| CAMC15.040 |
Microsatellite |
20.39 |
ATGGTGGTAATCACGGTGCAA |
TCCCCCATTATTGGATGGCCT |
- |
| CAMC15.009 |
Microsatellite |
20.9 |
TGGCTCAGCGGTTTATTGCC |
ACTTCTGCTGGTTGGACAGG |
- |
| CAMC15.041 |
Microsatellite |
21.05 |
CAGGTGTGAGTTGTGGGTCTT |
AGCTGTTCTGTGGGGTGCTA |
- |
|
RPGRIP1_intron 2 |
Gene |
21.34 |
CCTTGTGTTCTCCTTAAGTC |
TGAGCTTTGTTTGCCTTTGG |
- |
| CAMC15.029 |
Microsatellite |
21.56 |
GCCTGGCTAGCTCAGTTGA |
AACCCAAAGCTGGGCTTAATC |
- |
| CAMC15.034 |
Microsatellite |
21.89 |
CATGTGCTTCCCAGTCCTTT |
ATAGATGGGGTGCCTGAGTG |
- |
| CAMC15.030 |
Microsatellite |
22.2 |
AAAGGAGGCCCGGATATTTAT |
TGAGCACCCCTTTACCATTC |
- |
| CAMC15.035 |
Microsatellite |
22.24 |
GAAAAGGAGTACCGCCACC |
GCTTGGGGGAAGAAACCTAC |
- |
| CAMC15.031 |
Microsatellite |
22.79 |
CAGCCAATTGTGGCTAGTGA |
TGCCCCAATCTACCTTTCTG |
- |
| CAMC15.013 |
Microsatellite |
23.28 |
AATGAGTCCTACATCAGGCTC |
GGACTCCATGTCAAGGGTTG |
- |
| CAMC15.032 |
Microsatellite |
23.95 |
TTCTCCCTCTGCCTGTGTC |
TCCTGAGTGCTAGGCAGTTT |
- |
| CAMC15.033 |
Microsatellite |
24.45 |
TCAAGACCCTCAAAGGCATAA |
GCACCCCGATGTTTATTGTC |
- |
| CAMC15.016 |
Microsatellite |
25.1 |
TGCTGATTAGGCTGCTCAAG |
AAACCAGCTGCTTGGGACAC |
- |
| CAMC15.019 |
Microsatellite |
26.24 |
TCTCCTTTGACCCAAATTCCC |
AGCACTCAGGTTCAAGAGCC |
- |
| CAMC15.023 |
Microsatellite |
28.35 |
TGTCTCCTGAGGCATGGGA |
TCAGGTCCTCTCCCCATTTT |
- |
| FH3813 |
Microsatellite |
28.91 |
GATAGAGCTCCGTATCATGCTC |
TCTTTGATCAACTACCTCATGG |
[46] |
| CAMC15.027 | Microsatellite | 30.25 | TGAACATAGGGGAAGGGAGG | TCCCCTCCAGCAGTATGCA | - |
RPGRIP1 primers used to identify the presence of the insertion allele (*) and primers for polymorphic markers used for genotyping of the initially mapped cord1 locus on CFA15 are shown. Positions correspond to CanFam2.0 (accessed Oct 2008).