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. 2009 Oct 13;5:315. doi: 10.1038/msb.2009.72

Figure 1.

Figure 1

Schematic of an integrated, multi-dimensional approach to identify signaling pathway components. Genes harboring cell signaling-associated domains were randomly selected and then used to generate libraries of corresponding Flag-tagged cDNAs and siRNAs. These were then used in three high-throughput screens to map protein–protein interactions by LUMIER and to examine the effect of altering protein expression levels on a transcriptional reporter. The data from the three screens were integrated to derive a combined pathway score (CPS), which predicts the likelihood that a protein is a component of the signaling pathway being studied.

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