Figure 1.

Modulating the expression level of the scaffold Ste5. (A) The Ste5 scaffold and the pheromone MAP kinase pathway in Sacchaormyces cerevisiae. Ste5 binds to Ste4, Ste11, Ste7 and the MAP kinase, Fus3. Another MAP kinase, Kss1 (not depicted for clarity), also binds Ste5 and is also activated by Ste7. Upon pheromone stimulation, Ste5 facilitates signal transmission from Ste4 to Fus3/Kss1. Active Fus3 and Kss1 trigger the transcription of FUS1, cell-cycle arrest and ultimately mating. (B) The relative expression levels of Ste5 in strains expressing Myc-tagged Ste5 behind a constitutive promoter (pCYC, pADH, pTEF or pGPD) or the wild-type STE5 promoter (pSTE5). Error bars denote s.e.m. (n=3). Source data is available for this figure at www.nature.com/msb