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. Author manuscript; available in PMC: 2010 Sep 11.
Published in final edited form as: Circ Res. 2009 Aug 6;105(6):575–584. doi: 10.1161/CIRCRESAHA.109.202333

Figure 5. Extracellular HSP70 enhances BMP-induced condensation formation, ALP activity and mineralization in CVC.

Figure 5

CVC were treated with BMP-4 (50 ng/ml) or BMP-2 (300 ng/ml), HSP70 (50 ng/ml) and conditioned medium from BAEC transfected with the N-FLAG-MGP (approximately 50 ng/ml) construct or empty vector as indicated for 2 or 8 days. After 2 days, formation of condensations was examined (A), and ALP activity was determined (B, D). After 8 days, accumulation of calcium was determined (C, E).

Asterisks indicate statistically significant differences compared to control (empty plasmid). *, p<0.05; **, p < 0.01; ***, p < 0.001; Tukey’s test.

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