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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: J Neuropathol Exp Neurol. 2009 Mar;68(3):241–249. doi: 10.1097/NEN.0b013e3181996bfe

Figure 6.

Figure 6

Routine light microscopy (LM) and electron microscopy (EM) confirm axonal localization of calpain-mediated spectrin proteolysis (CMSP). (A, B) High magnification LM images of SBDP145 immunostaining in the thalamus suggest localization of CMSP to damaged axons and associated axonal swellings. The linear orientation of SBDP145 immunoreactivity in B suggests debris fields of degenerating axons. (C-E) SBDP145-immunostained sections processed for EM confirm localization of immunoreactivity in damaged axons and associated axonal swellings. Electron dense SBDP145-immunoreactivity (arrows) in a representative photomicrograph of a damaged thalamic axon exhibits a disrupted myelin sheath and cytoskeletal perturbation (C). Damaged axons display more mature bulb-like axon swellings characterized by pooling of intra-axonal organelles (D, E). Note the presence of SBDP145-immunoreactivity (arrows) within these swellings. Scale bar = 20 μm in A, B, 1 μm in C; 2 μm in D and E.