Fig. 4.

Assessing substrate specificity by in vitro translation of Ero1α, β1 integrin or LDLR. mRNA encoding Ero1α (A), β1 integrin (B), or LDLR (C) was translated in the presence of SP cells prepared from either HT1080 cells (lanes 1 and 8) or cells expressing the substrate-trapping mutants of the PDI family members as indicated (lanes 2-7 and 9-14). Following translation for 2 hours, SP cells were isolated and products of translation were either separated by non-reducing SDS-PAGE carried out immediately (totals; lanes 1-7) or following immunoisolation with V5-agarose (V5-IP; lanes 8-14). Radiolabelled proteins were visualised following autoradiography.