Figure 1.
Flow cytometry analysis of the sensitive strain. 106 cells ml-1 treated with 25 μg Kpkt for 24 h at 20°C, showing propidium iodide stained DBVPG 6500: (a) spheroplasts with heat-inactivated Kpkt; (b) spheroplast with Kpkt; and (c) sensitive whole cells with Kpkt. The peak on the left on the x axis is made up of viable spheroplasts/cell (not permeable to propidium iodide). The peak on the right of the x axis is made up of dead spheroplasts/cells (permeable to propidium iodide). Percentages represent the mortality after flow-cytometry analyses.