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Calcofluor White® staining for cell-wall damage. (a) untreated cells; (b) cells heat treated at 100°C for 10 min; (c) cells treated with 70% ethanol for 3 h; (d) cells broken after treatment for 2 h at 37°C with 0.02 UI of zymolyase; (e) cells treated for 24 h with 50 μg purified Kpkt; and (f) cells treated for 24 h with partially purified K1.
