Table 1.
Effects of the β-glucanase inhibitor castanospermine on Kpkt killer activity.
| Purified Kpkt | Enzymatic activity (g l-1 glucose) |
Killer activity Ø halo in well test (mm) |
Viable cell counts CFU ml-1 (sensitive cells) |
|---|---|---|---|
| No castanospermine | 0.032 | 11.0 | 0.5 × 102 |
| 25 μM castanospermine | 0.0001 | 0.0 | 1.5 × 105 |
The Kpkt treatment was carried out with 25 μg toxin for 24 h at 25°C. The initial inoculum of the sensitive strain was 105 cell ml-1. Growth of the sensitive cells was measured through viable plate counts.