Table 1.
Fluorescence intensity (lux; mean ± SE) and percentage of expression of EGF, TGFα, and EGFR.
| Embryos derived through natural mating |
Cloned embryos | ||||||
| Control treatments |
siRNA treatments |
||||||
| Embryonic stage | Positive | Negative | EGF | TGFα | EGFR | EGF + TGFα | Untreated |
| 1-cell | 59.3 ± 0.4a (100%) | 58.9 ± 0.6a (100%) | 42.7 ± 0.5b (72%) | 41.8 ± 1.1b (71%) | 42.6 ± 0.6b (73%) | 41.2 ± 0.7b (70%) | 57.9 ± 0.9a (98%) |
| 2-cell | 47.1 ± 0.6b (100%) | 47.2 ± 0.8b (100%) | 17.2 ± 0.7c (36%) | 16.6 ± 0.9c (35%) | 17.4 ± 0.7c (37%) | 16.3 ± 0.7c (35%) | 17.7 ± 0.6c (38%) |
| Morula | 67.5 ± 0.6d (100%) | 67.6 ± 0.7d (100%) | 32.1 ± 0.9e (48%) | 31.4 ± 0.6e (46%) | 31.7 ± 0.5e (47%) | 30.7 ± 0.6e (45%) | 34.5 ± 0.8e (51%) |
| Early blastocyst | 81.0 ± 1.0f (100%) | 80.9 ± 1.0f (100%) | 41.1 ± 0.7b (51%) | 40.5 ± 1.0b (50%) | 41.6 ± 0.7b (51%) | 39.8 ± 1.2b (49%) | 43.3 ± 1.0b (53%) |
| Late blastocyst | 84.4 ± 0.4g (100%) | 84.6 ± 0.5g (100%) | 67.5 ± 0.5d (80%) | 66.2 ± 0.6d (78%) | 66.8 ± 1.1d (79%) | 65.5 ± 0.6d (77%) | 64.8 ± 0.9d (77%) |
Means with different superscripts in the columns and rows are significantly different (P < 0.01) from values for control treatments. Percentages are relative to values for control treatments (positive, transfection with non-inhibitory siRNA; negative, no transfection or injection of PBS).