Figure 4.
Insulin IND treatment does not affect glomerular position, diameter, or cross-sectional area. A–D, Representative whole-mount images of awake M72TauLacZ mice IND treated as in Figure 3. The inset box represents area of tracked M72 glomerulus after coronal sectioning and counterstaining staining with neutral red as shown in single prime lettering, respectively (A1, B1, C1, D1). Double prime lettering (A2, B2, C2, D2) are representative M72-positive OSNs sampled in the epithelium from animals in the respective treatment groups. Mice (N = 4 per treatment group) were IND-treated for 7 d. Note: No gross change in OSN morphology or axonal targeting to the M72 specific glomerulus was apparent. Histogram summary of the mean (±SEM) glomerular diameter (E) or cross-sectional area (F) of the identified M72 glomerulus as in A1, B1, C1, and D1. Not significantly different, one-way ANOVA with a Student–Newman–Keuls post hoc test (α = 0.05). Scale bar: A–D, 1 mm; A1–D1, 50 μm; A2–D2, 10 μm.