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. 2009 Nov 16;187(4):497–511. doi: 10.1083/jcb.200905114

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© 2009 Geymonat et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Distribution of Bfa1 at SPBs is Tem1 independent. (A) Bfa1-GFP localization in MGY504 (tem1Δ BFA1-GFP dbf2 HyA) cells in metaphase–early anaphase and late anaphase cultivated at 30°C. (B) Bfa1-GFP localization in anaphase cells with a mispositioned spindle. Strain MGY547 (tem1Δ dyn1Δ BFA1-GFP dbf2 HyA) was cultivated at 14°C to increase the proportion of cells containing a mispositioned spindle. (A and B) Cells are outlined in white. (C) The ratios of Bfa1 signal intensities at SPBs were recorded in cells observed in A and B (n > 100). Bars, 5 µm.