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. 2009 Nov 16;187(4):481–496. doi: 10.1083/jcb.200907029

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© 2009 Llères et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — In vivo FLIM–FRET assay for chromatin compaction. (A) HeLa cells coexpressing either free EGFP and mCherry (top) or EGFP-fused to mCherry through a 7-aa linker (bottom) were imaged by multiphoton laser-scanning microscopy (MPLSM). The spatial distribution of the mean fluorescence lifetime of the EGFP donor (τ map) is displayed using a continuous pseudocolor scale ranging from 1.8 to 2.4 ns. (B) Representation of the FLIM–FRET chromatin compaction assay. Bars, 10 µm.