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. 2009 Nov 2;187(3):375–384. doi: 10.1083/jcb.200904140

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© 2009 Loo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Population-averaged levels of adipocyte markers increased monotonically on differentiating 3T3-L1 preadipocytes. (a) Western blots of differentiating 3T3-L1 cells probed with antibodies for AdipoQ, PPARγ, and HSL. Equal amounts of proteins were collected from each day of differentiation. For AdipoQ, only the tetramer bands are shown (D0, right before differentiation induction; D3–12, D3–12 after differentiation induction). (b) Bright field differential interference contrast and immunofluorescence images showing differentiating 3T3-L1 cells. For visualization only, marker intensity ranges were equalized across all images. (c) Comparisons of marker levels obtained from Western blots and median cell expression values from immunofluorescence images (see Materials and methods). Each graph was normalized to have unit sum across all days. Bar, 150 µm.

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