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. 2009 Nov 2;187(3):375–384. doi: 10.1083/jcb.200904140

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© 2009 Loo et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Temporal ordering of the identified 3T3-L1 subpopulations. (a) Changes in the percentages of subpopulations from D3–12. (b) Differentiating 3T3-L1 cells with visible lipid accumulations were tracked for 18 d using live cell phase contrast microscopy. Significant cell morphological changes for one of the 40 tracked cells are shown. Time-lapse videos for this and two other cells are shown in Video 1. (c) Examples of fixed 3T3-L1 cells in differential interference contrast (top) and immunofluorescence (bottom) from D6 to 12 that appeared similar to the cell tracked in b. (d) Bar chart showing the subpopulation assignments for all 40 tracked live cell sequences. Triangles indicate cells shown in Video 1. “Lost” refers to cells that either lost their LDs or disappeared from our view. Error bars indicate SEM (n = 3). Bars, 20 µm.

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