Skip to main content
. 2009 Nov 2;187(3):355–363. doi: 10.1083/jcb.200905031

Figure 2.

Figure 2.

Ana3 overexpression does not drive centrosome amplification or de novo formation. (A) Centriole numbers (marked by GFP-PACT) in WT and Ana3-overexpressing G2 primary spermatocytes. Centrioles were counted in a total of 140 WT and 203 Ubq–Ana3-GFP cells from five testes per condition. (B) Centrosome numbers (identified by colocalization of D-PLP and Cnn) in WT and Ana3-overexpressing prophase neuroblasts. Centrosomes were counted in a total of 41 WT and 38 Ubq–Ana3-GFP cells from four brains per condition. (C and D) Unfertilized eggs laid by UAS–Sak-GFP (C) and UAS–Ana3-GFP (D) mothers stained for tubulin (red) and D-PLP (blue). The high level overexpression of Sak, like the other core duplication proteins, induces de novo assembly of centriole-like structures, which nucleate MT asters (magnified in C’). In contrast, high level overexpression of Ana3 does not induce de novo centriole formation, and the only MTs visible are those around the polar bodies (C’ and D’, arrows). Bars: (C and D) 100 µm; (C’ and D’) 20 µm.