Fig. 4.
Hepatocytes (6 × 106) were incubated with or without CRLPs derived from palm, olive, or corn oil (0.3 μmol TG per milliliter) for 5 hours; the medium was then removed and replaced with fresh medium without CRLPs; and the incubation was continued for a further 16 hours. After this time, the medium was collected; and the content of apo B48 was determined. A, Apolipoprotein B48 bands in a typical experiment. B, Quantification by Western blotting coupled to ECL. Data are expressed as the percentage of the control in arbitrary units per milligram cell protein and are the mean ± SEM from 8 separate hepatocyte preparations, each performed in duplicate. Lane 1, positive control containing apo B48 and apo B100; lane 2, palm CRLPs; lane 3, olive CRLPs; lane 4, corn CRLPs; lane 5, without CRLPs.