Table 1.
Summary of the ‘dot-counting’ replication timing assay carried out on five different genes (PCDH11, ATRX, XIST, ZFX and autosomal control probe) in different human and gorilla cell types
| SS | DD | SD | SD% | N | Singlets | Doublets | |
|---|---|---|---|---|---|---|---|
| PCDH | |||||||
| ♀ blood | 48 | 16 | 37 | 36% | 101 | 66% | 34% |
| ♀ line 1 | 58 | 11 | 40 | 37% | 109 | 72% | 28% |
| ♀ line 2 | 40 | 25 | 39 | 37% | 104 | 57% | 45% |
| ♀ line ICF | 45 | 16 | 39 | 39% | 100 | 65% | 35% |
| ♀ Gorilla | 41 | 23 | 38 | 37% | 102 | 59% | 41% |
| ♀ neurobl. | 40 | 26 | 40 | 38% | 106 | 57% | 43% |
| ♀ neurobl.dif. | 93 | 21 | 44 | 28% | 158 | 73% | 27% |
| ♀ cortical | 61 | 11 | 30 | 29% | 102 | 75% | 25% |
| ♂ blood | 66 | 19 | 36 | 30% | 121 | 69% | 31% |
| ♂ line | 51 | 10 | 43 | 41% | 104 | 70% | 30% |
| ATRX | |||||||
| ♀ blood | 50 | 11 | 45 | 42% | 106 | 68% | 32% |
| ♀ line 1 | 62 | 20 | 48 | 37% | 130 | 66% | 34% |
| ♀ line 2 | 52 | 19 | 38 | 35% | 109 | 65% | 35% |
| ♀ line ICF | 51 | 15 | 35 | 35% | 101 | 68% | 32% |
| ♀ Gorilla | 49 | 14 | 39 | 38% | 102 | 67% | 33% |
| ♀ neurobl. | 62 | 12 | 46 | 38% | 120 | 71% | 29% |
| ♀ neurobl.dif. | 48 | 17 | 44 | 40% | 109 | 64% | 36% |
| XIST | |||||||
| ♀ blood | 67 | 8 | 34 | 31% | 109 | 77% | 23% |
| ♀ line 1 | 49 | 13 | 41 | 40% | 103 | 67% | 33% |
| ♀ line 2 | 61 | 14 | 34 | 31% | 109 | 72% | 28% |
| ♀ Gorilla | 53 | 10 | 43 | 40% | 106 | 70% | 30% |
| ♀ neurobl. | 66 | 20 | 39 | 31% | 125 | 68% | 32% |
| ♀ neurobl.dif. | 63 | 15 | 49 | 38% | 127 | 69% | 31% |
| ZFX | |||||||
| ♀ blood | 55 | 19 | 37 | 33% | 111 | 66% | 34% |
| ♀ line 1 | 56 | 21 | 35 | 31% | 112 | 66% | 34% |
| ♀ line 2 | 61 | 13 | 40 | 35% | 114 | 71% | 29% |
| ♀ Gorilla | 48 | 21 | 36 | 34% | 105 | 63% | 37% |
| ♀ neurobl. | 66 | 21 | 34 | 28% | 121 | 69% | 31% |
| ♀ neurobl.dif. | 77 | 13 | 30 | 25% | 120 | 77% | 23% |
| Autosom. | |||||||
| ♀ blood | 84 | 6 | 25 | 22% | 115 | 84% | 16% |
| ♀ neurobl.dif. | 77 | 7 | 33 | 28% | 117 | 80% | 20% |
| ♂ blood | 86 | 2 | 28 | 24% | 116 | 86% | 14% |
| BrdU-Lab. | |||||||
| PCDH | 47 | 8 | 54 | 50% | 109 | n.a. | n.a. |
| ATRX | 40 | 12 | 51 | 49% | 103 | n.a. | n.a. |
| Xist | 41 | 18 | 47 | 44% | 106 | n.a. | n.a. |
| ZFX | 73 | 36 | 100 | 48% | 209 | n.a. | n.a. |
| autosom. | 44 | 19 | 48 | 43% | 111 | n.a. | n.a. |
‘SS’ is the number of single-single hybridization patterns observed in each experiment, ‘DD’ is the number of double-double hybridization patterns observed in each experiment and ‘D’ is the number of single-double hybridization patterns observed in each experiment (also shown as a percentage), while ‘N’ is the total number of nuclei analysed for each experiment. ‘BrdU-labelled’ indicates the observations carried out on BrdU-positive nuclei. Percentages of total number of single signals (‘Singlets’) and double signals (‘Doublets’) are also included.