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. 2009 Dec 1;4(12):e8040. doi: 10.1371/journal.pone.0008040

Figure 6. Application of the simulation model in PKR over-expression.

Figure 6

A) In silico perturbation. Simulation of PKR over-expression and IKK or JNK inhibition with PKR over-expressed. Simulations are based upon the model including the JNK and IKK pathways but without the ERK feedback. B) Western blot results of the tyrosine and serine phosphorylation of IRS1 and the phosphorylation of PKR, after 15 mins of 1 nM insulin treatment, when PKR is over-expressed in HepG2 cells, with or without the IKK/JNK inhibition. IKK and JNK restores Tyr and reduces Ser phosphorylation of IRS1 (red box), as compared to the control with PKR over-expressed (blue box).