Fig. 3. Predicted and observed nucleosome distribution patterns on human β-globin DNA.

Panel (a) shows an overview of the 82-kb human β-globin locus, highlighting details of the 23-kb fragment (chr11 5,205,941–5,229,259) used in this study. Also shown are the in vivo nucleosome positions (purple) from Schones et al.⁵³, and the ~7.2-kb deletion spanning most of the δ-globin gene and ~6-kb of upstream sequence found in Corfu δβ-thalassemia patients. Major ticks are at 1-kb intervals, and minor ticks are in 200-bp subdivisions. The nucleosome distribution patterns predicted by the Segal et al.⁶ (magenta) and Field et al.¹⁴ (green) models for the 23-kb fragment are shown in (b), along with the observed patterns of nucleosome deposition determined by single-molecule imaging (blue). Panel (c) shows Pearson correlation analysis of the observed data for the β-globin locus compared results from the Segal et al.⁶ (left panel) and Field et al.¹⁴ models (right panel).