Fig. 1.

Translocation of GFP-labelled biosensors in response to mACh receptor activation in SH-SY5Y cells. Representative traces and confocal images of SH-SY5Y cells expressing eGFP-PH (a), GFP-Tubby (b) or eGFP-C1(2) (c) in response to MCh (100 μM; black horizontal bars). Data were expressed as a ratio change in cytosolic fluorescence emission (F) relative to the initial basal fluorescence (F₀). Data were represented as (F/F₀) − 1 (for eGFP-PH and GFP-Tubby) or 1 − (F/F₀) [for eGFP-C1(2)]. Scale bars, 10 μm.