Fig. 3.

The effect of PI4-kinase inhibition on translocation of GFP-labelled biosensors in response to MCh in SH-SY5Y cells. Representative images (i) and traces (ii) of eGFP-PH (a) and GFP-Tubby (b) translocation in response to MCh (1 mM; black horizontal bar) in cells in the presence of pre-incubated 1 μM (black trace) or 10 μM (red trace) wortmannin (as indicated by grey horizontal bar). Data were expressed as a ratio change in cytosolic fluorescence emission (F) relative to the initial basal fluorescence (F₀) and are representative of at least eight cells from three or more coverslips. Scale bars, 10 μm.