Fig. 5.

The effect of PI4-kinase inhibition on translocation of GFP-labelled biosensors in response to MCh in cultured neonatal rat hippocampal neurons. Representative images (i) and traces (ii) of eGFP-PH (a) and GFP-Tubby (b) translocation, in response to MCh (1 mM; black horizontal bar), in cultured rat hippocampal neurons (15 DIV) in the absence and presence of pre-incubated wortmannin (10 μM; grey horizontal bar). Data were expressed as a ratio change in cytosolic fluorescence emission (F) relative to the initial basal fluorescence (F₀) and are representative of at least 11 cells from three or more coverslips. Scale bars, 10 μm.