Figure 1.
top panel: A view from the posterior pole of an isolated rabbit lens with two sectors of the ciliary body glued in place on a clear polypropylene sheet. The sheet rests upon a thin metal frame that is held in place to the bottom of a 300 cm3 bathing chamber with molten paraffin that is allowed to harden (≈ 5 min) before the introduction of bathing medium. When viewed from above, the lens appears cloudy due to the paraffin below the plane of the polypropylene sheet. Middle panel: A profile of the isolated lens with the two sectors of ciliary body attached to the polypropylene sheet, as shown in the top panel. The ciliary body sectors are oriented along the visual axis of the camera so that the ED can be easily visualized, and thus only the sector nearest the camera is seen in the picture as a white wedge on the lens. To the right of the preparation is a ruler situated at the same focal plane as the lens for establishing the conversion ratio of the lens parameters (A-P length and ED) from pixels to metric units. Further to the right, the wall of the chamber within which the lens was immersed can be seen. Bottom panel: Shows the photo of the middle panel after the superimposition of an ellipsoid demarcation to highlight the lens outline from which the pixel dimensions of the A-P length and ED is easily obtained as described in Methods.