Figure 3. Effect of SP domain mutations on Gro/TLE1 interaction with Hes1 and Engrailed1.

FLAG epitope-tagged wild type (WT) or mutated Gro/TLE1 proteins were co-expressed in HEK293 cells with FLAG-Hes1 (A) or HA epitope-tagged En1 (B), as indicated. Each cell lysate (INPUT) was subjected to immunoprecipitation (IP) with either anti-Gro/TLE1 (A) or anti-HA (B) antibodies. Immunoprecipitates, together with 1/10 of each input lysate, were fractionated on a 10% SDS polyacrylamide gel, followed by Western blotting (WB) with the indicated antibodies.