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. 2009 Dec 1;4(12):e8108. doi: 10.1371/journal.pone.0008108

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A fifty percent reduction in the concentration of topically applied rabbit antibodies was detected in vaginal washes collected 1 hr post-inoculation compared to a 2 min time point. Antibody levels decreased further over time. The limit of detection was 7.8 ng/ml. (A) Concentration of rabbit IgG (µg/ml) in mouse vaginal washes was determined in duplicate by capture ELISA. (B) The percent of antibody remaining relative to the average 2 minute value is shown for each time point. Results are combined from two experiments and each animal was used for a single time point (n = 2–3 mice per time point).