Skip to main content
. 2000 Aug 29;97(18):10185–10190. doi: 10.1073/pnas.97.18.10185

Figure 6.

Figure 6

Analysis of the antisense effect on proliferative shutoff. A4 cells were exposed to100 pM estradiol to allow for maximal proliferation in all of the experimental groups. Tet and R1881 were added as described below. Cells were stained for BrdUrd (green fluorescence) and Hoechst 33258 DNA stain (blue). (A) Maximal cell proliferation control (no R1881 added, tet added). (B) Androgen-induced shutoff without antisense AS3 induction (R1881 added, tet added). (C) Antisense AS3 induction (no R1881 added, tet withdrawn). (D) The effect of antisense induction on the androgen-induced shutoff (R1881 added, tet withdrawn). The photomicrographs were taken at ×200 by using standard FITC filters for BrdUrd detection and UV filters for Hoechst 33258 detection.