Skip to main content
. 2000 Aug 29;97(18):10185–10190. doi: 10.1073/pnas.97.18.10185

Figure 7.

Figure 7

Analysis of the effect of sense AS3 expression on proliferation. S9 cells were treated with tet and hormones as described below and stained for BrdUrd (green fluorescence) and Hoechst 33258 nuclear stain (blue). (A) Maximal cell proliferation control (no R1881 added, tet added). (B) Androgen-induced shutoff control without AS3 induction (R1881 added, tet added). (C) AS3 induction by tet withdrawal (no R1881 added, tet withdrawn). (D) Androgen-induced shutoff and AS3 induction by tet withdrawal (R1881 added, tet withdrawn). The photomicrographs were taken ×200 by using standard FITC filters for BrdUrd detection and UV filters for Hoechst 33258 detection.