FIGURE 3.

Effect of buffer conditions on the stability of full-length transcripts. The relative levels of the 5′ and 3′ ends of the mRNA for each of the genes, Actb (A), Gapdh (B), Th (C), and Slc6a3 (D), were determined. As in Figure 2, data are expressed as the means (+SEM) of the ΔΔCt, with the 5′–3′ ΔCt for each buffer condition normalized to the 5′–3′ ΔCt for the fresh-frozen condition. Again, only the overnight (o/n) high salt incubations, and the 2M NaCl buffer in particular, resulted in transcript completeness unchanged from that observed with the fresh-frozen condition. * P < 0.05, ** P < 0.01, n ≥ 5.