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Chemical probing of the pK1, pK4, and helix 2 tmRNA region in the ribosomal complexes (Rib) and in the solution (Sol) for tmRNA-2 (2), tmRNA-4 (4), tmRNA-5 (5), and tmRNA-11 (11) by KE (A), CMCT (B,C,E), and DMS (D). Dideoxy sequencing lanes are indicated by T, G, C, and A. The sequence of tmRNA is shown on the right side of the gels. Arrows indicate the position of nucleotides which displayed different accessibility to modifying reagents in the complexes and in solution.
