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. 2009 Dec;15(12):2321–2330. doi: 10.1261/rna.1710309

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FIGURE 6. — DNA templates used in transcription experiments. (a) Plasmid pZ-NCRrnl, containing the entire noncoding region (NCR, 1913 bp) and a portion (398 bp) of the downstream rnl gene cloned into pZErO-2; (b) pZ-rnl, containing only the portion of the rnl gene but no upstream sequences; (c) pBS-rnl, containing the same portion of the rnl gene cloned into pBluescriptSK⁺ for in vitro expression by T7 RNA polymerase from the T7 promoter (T7P). Relevant restriction enzyme recognition sites are indicated.