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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Mol Microbiol. 2009 Apr 21;72(4):1022–1036. doi: 10.1111/j.1365-2958.2009.06703.x

Figure 1.

Figure 1

Estimation of altruistic suicide in enterococcal populations by flow cytometry. A. Representative flow histograms of GBAP responders from wild type VT15, and isogenic protease mutants- VT16 (ΔgelE), VT17 (ΔsprE) and VT18 (ΔgelEsprE) cultured to stationary phase. The pVT31 reporter plasmid in these isogenic strains activates GFP expression in response to GBAP quorum signaling. GFP fluorescence (FL-1H) corresponding to VT15, VT16, VT17 and VT18 are plotted (thick solid lines) and are contrasted in each instance with autofluorescence (thin solid lines) from VT14 (V583 pVT30, reporter construct lacking functional gelE promoter fusion). B. Percent GFP+ cells (GBAP responders) following overnight growth of VT13 (V583::fsrA), VT14, VT15, VT16, VT17 and VT18. The percent estimates were derived from histograms of seven independent trials ± SE using the CELLQuest program (version 3.1, Beckton and Dickinson).