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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Immunol. 2009 Aug 10;183(5):3237–3248. doi: 10.4049/jimmunol.0901548

Figure 3.

Figure 3

Immunization with alum adjuvant mobilizes CD93+ cells from the BM and localizes them in splenic bridging channels. Mice were immunized with NP-CGG/alum via i.p. and 16 days after immunization, the location of mobilized CD93+ BM cells was determined by immunofluorescence microscopy. Frozen spleen sections from naïve (A and D) and immunized (B, C, E, F) BL/6 (A–C) or CD154−/− (D–F) mice were labeled with mAb specific for IgM (blue; AlexaFluor350), TCRβ (green; FITC) and CD93 (red; PE). Arrows indicate CD93+ lymphocyte-like cells. Bridging channel areas indicated by rectangles in panels B and E are shown at higher magnification in panels C and F, respectively. Original magnification: ×100. Data shown are typical of at least two independent experiments.