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. Author manuscript; available in PMC: 2010 Apr 16.
Published in final edited form as: Cell. 2009 Oct 16;139(2):285–298. doi: 10.1016/j.cell.2009.07.047

Figure 6.

Figure 6

Fz4−/− REC lines exhibit defects in the formation of capillary-like structures

(A) Diagram of immuno-affinity purification of vascular fragments (upper), and immunohistochemical characterization of isolated fragments (lower; scale bar 20 um) and cloned REC lines (right; scale bar, 50 um).

(B) Scratch-induced motility on gelatin-coated dishes. Left, phase contrast images zero and 24 hours after the scratch; right, quantification of cell motility. Scale bar, 200 um.

(C) WT, Lrp5−/−, and Ndp− REC lines form capillary-like structures six hours after plating on Matrigel; Fz4−/− REC lines do not. Scale bar, 200 um.

(D) Matrigel cultures of diI-labeled WT and diO-labeled Fz4−/− RECs, mixed immediately before plating at the indicated ratios. Bottom, Matrigel cultures of the pure RECs. Scale bar, 100 um.