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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Nat Biotechnol. 2009 Nov 1;27(11):1025–1031. doi: 10.1038/nbt.1583

Figure 3. Normalized Coverage Distribution Plots.

Figure 3

The validation phase 457 amplicons amplified by traditional PCR (A) and microdroplet PCR (B) and the scale-up phase 3976 amplicons amplified by microdroplet PCR (C). Normalized coverage is the absolute base coverage divided by the mean coverage of bases for the indicated sample. Each colored line represents either one of the six samples (A & B) or one of two sequencing platforms (C). The solid colored lines represent the cumulative distribution (left axis) for each sample. The colored dashed lines indicate a skewed normal distribution (right axis) for each sample. For each sample the mean coverage across all bases are listed.