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. 2009 Nov 1;23(21):2496–2506. doi: 10.1101/gad.1825209

Figure 3.

Figure 3.

The plant proteasome, whose catalytic subunit (PBA1) exhibits a caspase-3-like (DEVDase) activity, is required for bacterially induced hypersensitive cell death in leaves. (A–D) Trypan-blue staining of dead cells of Arabidopsis leaves that were infected with Pst DC3000/avrRpm1 in the absence (A) or presence of caspase-3 inhibitor (B), an inhibitor to proteasome PBA1 subunit (C), and a general proteasome inhibitor (D). Bars, 100 μm. (E,F) Effects of proteinase inhibitors on DEVDase activity (E) and the PBA1 activity (F) in the infected leaves. Vertical bars indicate standard errors of three repeated experiments. (N.D.) Not detected. (G) The three ipba1 lines had reduced activities of not only the PBA1 subunit and DEVDase, but also the other proteasome subunits (PBB and PBE). Vertical bars indicate standard errors of three repeated experiments. (H) Correlation between the DEVDase and PBA1 activities in two or three plants of 17 ipba1 lines and wild type. (I) The extract of the infected leaves was pulled down with (+) or without (−) biotin-DEVD-fmk and were then subjected to immunoblot with anti-PBA1 antibody.