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. 2009 Nov 1;23(21):2551–2562. doi: 10.1101/gad.1839309

Figure 5.

Figure 5.

dTld binds BMP4 and BMP7. (A) Diagram of dTld and the dTld CUB1/2 and CUB4/5 constructs. (B) dTld CUB1/2ΔL mutant is not secreted, and CUB4/5SR mutant has impaired secretion in transfected 293T cells. Multiple bands are presumably due to glycosylation. (C) dTld CUB1/2 binds BMP4 within the physiological range (KD ≈ 17 nM). (D) When BMP7 is placed on the sensor chip, dTld CUB1/2 protein is able to bind to it (KD ≈ 26 nM). (E,F) Both dTld CUB4/5 and CUB4/5SR bind BMP4 with similar KD. (G–J) dTld CUB1/2 (n = 81, 78% dorsalized) and CUB4/5 (n = 79, 77% dorsalized) have anti-BMP (dorsalizing) activity in a Xenopus assay, while dTld CUB4/5SR did not show any anti-BMP activity (n = 52).