Figure 2. Capacitative Ca²⁺ entry (CCE) controls nuclear translocation of NFATc1 after store depletion with thapsigargin.

A: Representative CCE trace induced by 2 mM [Ca²⁺]_o in CPAE cells with depleted Ca²⁺ stores. Cells were pre-treated with 10 μM thapsigargin (TG) under Ca²⁺-free conditions to deplete the ER of Ca²⁺ (not shown). Application of 2 mM [Ca²⁺]_o for 5 min resulted in Ca²⁺-influx (grey trace), which induced nuclear translocation of NFATc1 (black trace). B: Summary data: CCE alone was sufficient to induce nuclear translocation of NFATc1-GFP in CPAE cells. This effect was dependent on CCE duration (black columns). CsA inhibited CCE-induced nuclear translocation of NFAT. Store depletion alone (TG, [Ca²⁺]_o = 0) without CCE failed to initiate NFAT translocation (grey columns). *, statistically significant (P < 0.05) compared to control.