Figure 2.

Cholesterol depletion does not change APP biosynthesis and secretion. Hippocampal neurons were grown for 4 days in the presence (+) or absence (−) of lovastatin/mevalonate. After infection with SFV/APP, cells were treated (+) or not (−) for 20 min with 5 mM methyl-β-cyclodextrin, and metabolically labeled for 2.5 hr. Cellular and secreted APP were recovered by immunoprecipitation.