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Cholesterol depletion reduces association of APP with detergent-insoluble low-density DIGs. Hippocampal neurons treated or not for 4 days with lovastatin/mevalonate and 20 min with 5 mM methyl-β-cyclodextrin were extracted on ice with 1% Triton X-100 immediately after pulse labeling for 20 min or after an additional chase for 100 min. After flotation in an OptiPrep step-gradient, APP was immunoprecipitated from the collected fractions.
