Figure 2.

RT-PCR amplification strategy of S. litura SlSCPx cDNA from mRNA. (A) RT-PCR amplification strategy, PCR-amplified products (B) and diagram of alternative splicing of SlSCPx transcripts (C). The positions of the forward and reverse primers for RT-PCR are indicated in arrows below the sequence diagram. The arrows above the sequence diagram show the position of primers for the SlSCPx dsRNA preparation (A). The two PCR products were amplified and their sizes were 1.6 kb for the full-length SlSCPx and 0.6 kb for the SlSCPx-C region, respectively (B). The dotted line shows the deleted SlSCPx-t fragmentfrom the N-terminal (43 to 1,168 nucleotides) of SlSCPx sequence, as compared to SlSCPx-2.