Figure 5.
Northern and western blotting analysis of temporal and spatial expression of the SlSCPx gene and protein. Ten microgram aliquots of total RNA from various tissues and stages was used in each of the lanes in the northern blots (A-C). The blots were hybridized with 32P-labelled DNA probes of the SlSCPx-2 region (A and B) and the SlSCPx-t region (C), respectively. In the western blots (D-F), the protein extracts were blotted with anti-SlSCPx-2 antibody (D and the lower panel of F) and anti-SlSCPx-t antibody (E and the upper panel of F), respectively. The arrowhead in (E) indicates an unidentified protein present in the fat body and epidermis. C: the recombinant protein extract from bacterial expression system. L: larvae; D: day; H: hours; WH: white head; MG: midgut; EP: epidermis; FB: fat body; P: pupae. Pro-SlSCPx is the putative pre-mutual transcript of the full-length SlSCPx mRNA.